Generator

Part:BBa_I756001:Design

Designed by: Andy Mendelsohn   Group: iGEM07_Bay_Area_RSI   (2007-05-26)


Eukaryotic Protein generator for DOCK11


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2745
    Illegal PstI site found at 2475
    Illegal PstI site found at 2803
    Illegal PstI site found at 3583
    Illegal PstI site found at 4017
    Illegal PstI site found at 6517
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2745
    Illegal NheI site found at 3822
    Illegal NheI site found at 6654
    Illegal PstI site found at 2475
    Illegal PstI site found at 2803
    Illegal PstI site found at 3583
    Illegal PstI site found at 4017
    Illegal PstI site found at 6517
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2745
    Illegal BglII site found at 926
    Illegal BglII site found at 3212
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2745
    Illegal PstI site found at 2475
    Illegal PstI site found at 2803
    Illegal PstI site found at 3583
    Illegal PstI site found at 4017
    Illegal PstI site found at 6517
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2745
    Illegal PstI site found at 2475
    Illegal PstI site found at 2803
    Illegal PstI site found at 3583
    Illegal PstI site found at 4017
    Illegal PstI site found at 6517
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 718
    Illegal BsaI.rc site found at 1142
    Illegal SapI site found at 2029
    Illegal SapI site found at 2068
    Illegal SapI site found at 4116
    Illegal SapI site found at 4803


Design Notes

want high expression


Source

from parts in registry

References